Acute gene inactivation in the adult mouse liver using the CRISPR-Cas9 technology

Xiao Wang; Bo-Lin Xu; Xiao-Wei Chen

doi:10.1016/j.xpro.2021.100611

Acute gene inactivation in the adult mouse liver using the CRISPR-Cas9 technology

STAR Protoc. 2021 Jun 17;2(3):100611. doi: 10.1016/j.xpro.2021.100611. eCollection 2021 Sep 17.

Authors

Xiao Wang¹, Bo-Lin Xu², Xiao-Wei Chen²

Affiliations

¹ School of Life Sciences, Peking University, Beijing 100871, China.
² Institute of Molecular Medicine, College of Future Technology, Peking University, Beijing 100871, China.

Abstract

Genetic manipulation in mice allows the discovery of gene function and biological mechanisms in vivo. The widely used Cre/LoxP system usually takes months to years especially when starting with the production of floxed alleles of a new gene of interest (GOI). Here, we describe a protocol using the CRISPR-Cas9 system to acutely inactivate the GOI in adult mice. This protocol enables hepatocyte-specific gene editing within 4 weeks in adult mice and avoids compensatory effects of traditional gene inactivation initiated during various developmental stages. For complete details on the use and execution of this protocol, please refer to Wang et al. (2020).

Keywords: CRISPR; Genetics; Model Organisms; Sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CRISPR-Cas Systems*
Gene Editing / methods
Gene Knockout Techniques
Liver / metabolism*
Mice
RNA, Guide, CRISPR-Cas Systems / genetics
Reproducibility of Results

Substances

RNA, Guide, CRISPR-Cas Systems