Evaluation of response to tyrosine kinase inhibitors in renal cell carcinoma patients based on expression of miR-99b, -144, -210, -222, -302а and -377 in tumor tissue

Exp Oncol. 2021 Jun;43(2):98-103. doi: 10.32471/exp-oncology.2312-8852.vol-43-no-2.16383.

Abstract

Background: Renal cell carcinoma (RCC) is one of the most common solid tumors in adults highly resistant to conventional therapies. The expression profile of a number of miRNAs correlates with RCC response to chemotherapeutic agents.

Aim: To identify the association of tumor miRNAs expression with neoadjuvant treatment response in patients with RCC.

Materials and methods: We analyzed the expression levels of tumor miR-99b, -144, -155, -210, -222, -302а, -377 in 93 RCC patients who received pazopanib or sunitinib in neoadjuvant regimen using RT-PCR. RNU48 was used as a reference miRNA.

Results: The levels of expression of miR-99b and -377 are associated with the RCC response to pazopanib, and microRNA-210 and -377 to sunitinib. The characteristic expression profile of miR-99b, -144, -222, -377, and miR-302a determined in 90% of cases was delineated in pazopanib responders as opposed to nonresponders. Similarly, the characteristic expression profile of miR-210, -222, -302a and -377 was suggested for sunitinib responders.

Conclusions: Levels of miR-99b, -210 and -377 expression in RCC tumor tissue might be used as a basis for future predictive panel intended for the assessment of the sensitivity to the regimens of neoadjuvant RCC treatment.

MeSH terms

  • Adult
  • Aged
  • Biomarkers, Tumor / genetics
  • Carcinoma, Renal Cell / drug therapy*
  • Carcinoma, Renal Cell / genetics
  • Drug Resistance, Neoplasm / genetics*
  • Female
  • Humans
  • Indazoles / therapeutic use
  • Kidney Neoplasms / drug therapy*
  • Kidney Neoplasms / genetics*
  • Male
  • MicroRNAs / biosynthesis*
  • Middle Aged
  • Protein Kinase Inhibitors / therapeutic use*
  • Pyrimidines / therapeutic use
  • Retrospective Studies
  • Sulfonamides / therapeutic use
  • Sunitinib / therapeutic use
  • Treatment Outcome

Substances

  • Biomarkers, Tumor
  • Indazoles
  • MicroRNAs
  • Protein Kinase Inhibitors
  • Pyrimidines
  • Sulfonamides
  • pazopanib
  • Sunitinib