Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research

Erica C Pandolfi; Timothy J Hunt; Sierra Goldsmith; Kellie Hurlbut; Sherman J Silber; Amander T Clark

doi:10.1016/j.scr.2021.102446

Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research

Stem Cell Res. 2021 Jul:54:102446. doi: 10.1016/j.scr.2021.102446. Epub 2021 Jun 24.

Authors

Erica C Pandolfi¹, Timothy J Hunt¹, Sierra Goldsmith², Kellie Hurlbut², Sherman J Silber², Amander T Clark³

Affiliations

¹ Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA.
² Infertility Center of St. Louis, St. Luke's Hospital, St. Louis, MO, USA.
³ Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA. Electronic address: [email protected].

Abstract

Three induced pluripotent stem cell sublines (hiPSCs) were generated from human dermal human dermal fibroblasts (HDFs) derived from a human skin punch biopsy. The biopsy was donated from a woman with known infertility due to ovarian failure. The hiPSC sublines were created using Sendai virus vectors and were positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA-4. Pluripotency was verified using PluriTest analysis and in vitro differentiation using Taqman Real-Time PCR assays for somatic lineage markers. This participant's monozygotic twin sister also donated a skin-punch biopsy, whose resulting hiPSC lines were published previously as a resource.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Cellular Reprogramming
Female
Fibroblasts
Humans
Induced Pluripotent Stem Cells*
Sendai virus
Skin

Abstract

Publication types

MeSH terms

Grants and funding