1,25-Dihydroxyvitamin D3 transcriptionally regulates tumour necrosis factor mRNA during HL-60 cell differentiation

Immunology. 1988 Jan;63(1):43-6.

Abstract

We investigated TNF-alpha gene expression in HL-60 cells under the influence of 1,25(OH)2D3. Our results show that TNF-alpha gene expression is transcriptionally activated by 1,25(OH)2D3. Despite the increase of TNF-alpha mRNA, very little TNF protein is found in either supernatants or cell lysates of HL-60 cells cultured in the presence of the vitamin. Therefore 1,25(OH)2D3 is regulating the transcription of the TNF-alpha gene in HL-60 cells without affecting translation. Our data suggest that an additional signal is necessary under physiological conditions to control post-transcriptionally TNF-alpha protein synthesis and secretion.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Calcitriol / physiology*
  • Cell Differentiation
  • Gene Expression Regulation
  • Humans
  • Leukemia, Myeloid, Acute / genetics*
  • Leukemia, Myeloid, Acute / immunology
  • Leukemia, Myeloid, Acute / pathology
  • RNA, Messenger / analysis*
  • Transcription, Genetic*
  • Tumor Cells, Cultured / cytology
  • Tumor Necrosis Factor-alpha / genetics*

Substances

  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • Calcitriol