Serum miR-224, miR-760, miR-483-5p, miR-378 and miR-375 as potential novel biomarkers in rheumatoid arthritis

Int J Clin Pract. 2021 Nov;75(11):e14651. doi: 10.1111/ijcp.14651. Epub 2021 Aug 8.

Abstract

Background: Rheumatoid arthritis (RA) is an inflammatory autoimmune disease which affects various tissues and organs mainly joints. Serum microRNAs are considered a new class of non-coding RNA which plays a vital role in pathogenesis of RA.

Methods: The current study was conducted on 80 RA patients and 80 healthy participants. Serum expression levels of miR-224, miR-760, miR-483-5p, miR-378 and miR-375 were evaluated via real-time quantitative polymerase chain reaction (PCR).

Results: Significant upregulation of miR-224, miR-760, miR-483-5p, miR-378 and miR-375 was reported in the present study with respect to the control group (P = .031, P = .017, P = .026, P = .036 and P = .05, respectively). Furthermore, significant positive correlation between the abovementioned microRNAs with DAS28 score (P < .001, each) was demonstrated.

Conclusion: Early detection of RA could be achieved through evaluation of serum expression of miR-224, miR-760, miR-483-5p, miR-378 and miR-375 which also may be used as targets for treatment of patients with RA.

MeSH terms

  • Arthritis, Rheumatoid* / genetics
  • Biomarkers
  • Humans
  • MicroRNAs* / genetics

Substances

  • Biomarkers
  • MIRN224 microRNA, human
  • MIRN375 microRNA, human
  • MIRN378 microRNA, human
  • MIRN483 microRNA, human
  • MIRN760 microRNA, human
  • MicroRNAs