Facile synthesis of core\shell Fe₃O₄@mSiO₂(Hb) and its application for organic wastewater treatment

Treatment of organic wastewater is a challenging task. Biological techniques using biocatalysts have shown their benefits in organic wastewater treatment. In this research, a novel biocatalyst was developed by encapsulation of Fe3O4 microspheres and haemoglobin (Hb) with mesoporous silica, named Fe3O4@mSiO2(Hb). Fe3O4@mSiO2(Hb) exhibited typical mesoporous characteristics (mesoporous silica), magnetic feature (Fe3O4) and peroxidase activity (Hb). The results showed that the immobilization of Hb into Fe3O4@mSiO2 did not affect its activity. In addition, Fe3O4@mSiO2(Hb) exhibited a higher efficiency in the peroxidation of aromatic compounds than free Hb. The peroxidase activity of the synthesized biocatalyst was estimated to be 120 Ug^-1, which was almost four times greater than that of previously reported immobilized Hb. Also, the Km of Fe₃O₄@mSiO₂(Hb) was similar to that of the free Hb and it was estimated to be 4.3 × 10^-4 μM, indicating that the activity of the Hb in the immobilized enzyme was not affected after immobilization. The immobilized enzyme was also found to be stable, recyclable and reusable. Taken together, these results indicate that the Fe₃O₄@mSiO₂(Hb) has good potential to be used for treating organic wastewater containing aromatic compounds. The magnetically separable novel biocatalyst developed in this study provided not only a more suitable microenvironment for retaining the activity of Hb, but also demonstrated enhanced stability and activity under unfavorable conditions.