HLA-B*27 typing using a triplex real time PCR in routine laboratory

Marte K Viken; Alice L Pedersen; Menaka Andersen; Tore Jensen; Benedicte A Lie; Line M L Boulland

doi:10.1111/tan.14386

HLA-B*27 typing using a triplex real time PCR in routine laboratory

HLA. 2021 Oct;98(4):366-369. doi: 10.1111/tan.14386. Epub 2021 Aug 6.

Authors

Marte K Viken¹, Alice L Pedersen¹, Menaka Andersen¹, Tore Jensen¹, Benedicte A Lie^{1

2}, Line M L Boulland¹

Affiliations

¹ Department of Immunology, Oslo University Hospital and University of Oslo, Oslo, Norway.
² Deptartment of medical genetics, Oslo University Hospital and University of Oslo, Oslo, Norway.

PMID: 34342381
DOI: 10.1111/tan.14386

Abstract

We have established an in-house HLA-B*27 multiplex typing assay by using a combination of previously published, newly designed and commercial primers and probes for use with real-time PCR instruments. Hence, facilitating quick and large-scale HLA-B*27 typing.

MeSH terms

Alleles
HLA-B Antigens* / genetics
Histocompatibility Testing
Humans
Laboratories*
Real-Time Polymerase Chain Reaction

Substances

HLA-B Antigens