Purification and characterization of a lectin from Phaseolus acutifolius var. latifolius

Prep Biochem. 1987;17(4):379-96. doi: 10.1080/00327488708062503.

Abstract

The lectin from Phaseolus acutifolius var. latifolius has been purified to homogeneity by affinity chromatography using stroma-sephadex, gel filtration and electrofocusing. The purified lectin consists of four subunits of 21 Kd molecular mass each one with 7.4% w/w total carbohydrate. Red cells of human and animal species, are agglutinated but are not inhibited by a series of sugars. Mitogenicity and immunosuppressive activities were demonstrated. Agglutination requires magnesium and calcium ions.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acids / analysis
  • Animals
  • Chromatography
  • Fabaceae / analysis*
  • Humans
  • Immunosuppressive Agents
  • In Vitro Techniques
  • Isoelectric Point
  • Lectins / isolation & purification*
  • Lectins / pharmacology
  • Lymphocyte Activation / drug effects
  • Molecular Weight
  • Phytohemagglutinins / isolation & purification*
  • Phytohemagglutinins / pharmacology
  • Plant Lectins
  • Plants, Medicinal*
  • Temperature

Substances

  • Amino Acids
  • Immunosuppressive Agents
  • Lectins
  • Phytohemagglutinins
  • Plant Lectins