Establishment of an Experimental System for Intraperitoneal Chemotherapy in a Rat Model

Sunwoo Park; Soo Jin Park; Hee Su Lee; Jiyeon Ham; Eun Ji Lee; Junsik Kim; Soomin Ryu; Aeran Seol; Whasun Lim; Jung Chan Lee; Gwonhwa Song; Hee Seung Kim; KoRIA Trial Group

doi:10.21873/invivo.12554

Establishment of an Experimental System for Intraperitoneal Chemotherapy in a Rat Model

In Vivo. 2021 Sep-Oct;35(5):2703-2710. doi: 10.21873/invivo.12554.

Authors

Sunwoo Park¹, Soo Jin Park², Hee Su Lee³, Jiyeon Ham¹, Eun Ji Lee², Junsik Kim³, Soomin Ryu¹, Aeran Seol², Whasun Lim⁴, Jung Chan Lee^{5

6}, Gwonhwa Song⁷, Hee Seung Kim⁸; KoRIA Trial Group

Affiliations

¹ Institute of Animal Molecular Biotechnology and Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
² Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Republic of Korea.
³ Interdisciplinary Program in Bioengineering, Seoul National University Graduate School, Seoul, Republic of Korea.
⁴ Department of Food and Nutrition, Kookmin University, Seoul, Republic of Korea.
⁵ Department of Biomedical Engineering, Seoul National University College of Medicine, Seoul, Republic of Korea.
⁶ Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University, Seoul, Republic of Korea.
⁷ Institute of Animal Molecular Biotechnology and Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea; [email protected] [email protected].
⁸ Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Republic of Korea; [email protected] [email protected].

Abstract

Aim: To establish an experimental system for comparing different methods of intraperitoneal chemotherapy in a rat model.

Materials and methods: We used six-week-old Sprague-Dawley rats, and created an early postoperative intraperitoneal chemotherapy (EPIC) system using 18-gauge syringes and evacuators, and a hyperthermic intraperitoneal chemotherapy (HIPEC) system using two peristaltic pumps which controlled the flow rate and temperature. Pressurized intraperitoneal aerosol chemotherapy (PIPAC) was achieved using a nozzle for dispersing aerosols at a flow rate up to 41.5 ml/min. The distribution and intensity of 0.2% trypan blue dye was compared among three methods.

Results: The distribution was limited and the intensity was weak after EPIC, and the dye stained moderately in gravity-dependent regions after HIPEC. On the other hand, the distribution was the most comprehensive, and the intensity was the greatest after PIPAC.

Conclusion: This experimental system in a rat model may reflect the comparative effect among EPIC, HIPEC and PIPAC in humans.

Keywords: Experimental system; intraperitoneal chemotherapy; rat.

MeSH terms

Aerosols
Animals
Hyperthermic Intraperitoneal Chemotherapy*
Rats
Rats, Sprague-Dawley

Substances

Aerosols