A Novel Tension Machine Promotes Bone Marrow Mesenchymal Stem Cell Osteoblastic and Fibroblastic Differentiation by Applying Cyclic Tension

Yi Zhao; Yiping Huang; Lingfei Jia; Ruoxi Wang; Kuang Tan; Weiran Li

doi:10.1155/2021/6647651

A Novel Tension Machine Promotes Bone Marrow Mesenchymal Stem Cell Osteoblastic and Fibroblastic Differentiation by Applying Cyclic Tension

Stem Cells Int. 2021 Aug 5:2021:6647651. doi: 10.1155/2021/6647651. eCollection 2021.

Authors

Yi Zhao¹, Yiping Huang¹, Lingfei Jia^{2

3}, Ruoxi Wang¹, Kuang Tan¹, Weiran Li¹

Affiliations

¹ Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, China.
² Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, China.
³ Central Laboratory, Peking University School and Hospital of Stomatology, Beijing 100081, China.

Abstract

Bone marrow mesenchymal stem cells (BMSCs) are intraosseous stem cells, and the effects of tensile strain on BMSC differentiation mediate several bone-related treatments. To study the response of BMSCs under tension, we designed and developed a small cellular tension instrument, iStrain. When iStrain applied tension on BMSCs, these cells exhibited convergence in the alignment direction and lengthening of the cell processes and cell body. Real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting demonstrated that iStrain-mediated cyclic tension promotes the differentiation of BMSCs toward osteogenesis and fibrogenesis. And the mRNA and protein expression of differentiation-related genes changes with the extension of tension time.