Abstract
A strain of Clostridium sporogenes, an anaerobic bacterium, isolated from sewage in New York City synthesizes two constitutive enzymes with action on steroid molecules: (i) an enzyme capable of selectively acetylating the 21-hydroxyl function of certain steroids and (ii) the corresponding esterase. Under our experimental conditions the enzymes have a strict structural requirement for 3-keto-4-ene and C-20-keto or 20 alpha-hydroxyl group and convert their respective substrates to a mixture of free and acetylated products.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetylation
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Adrenal Cortex Hormones / metabolism*
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Aldehyde-Lyases / metabolism
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Anaerobiosis
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Chromatography, Thin Layer
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Clostridium / metabolism*
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Cytochrome P-450 Enzyme System / metabolism
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Hydrolysis
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Kinetics
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Nitroblue Tetrazolium / metabolism
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Spectrophotometry, Ultraviolet
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Steroid 17-alpha-Hydroxylase
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Substrate Specificity
Substances
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Adrenal Cortex Hormones
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Nitroblue Tetrazolium
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Cytochrome P-450 Enzyme System
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Steroid 17-alpha-Hydroxylase
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Aldehyde-Lyases