The relationship between hepatitis B virus serum DNA, RNA and quantitative hepatitis B surface antigen, and the predictive value for mother-to-child transmission: an observational cohort study

BJOG. 2022 Jan;129(2):241-247. doi: 10.1111/1471-0528.16884. Epub 2021 Sep 21.

Abstract

Objective: To explore the relationships between hepatitis B virus (HBV) DNA, HBV RNA and hepatitis B surface antigen (HBsAg) and to evaluate their predictive value for mother-to-child transmission of HBV.

Design: An observational cohort study.

Setting: First Hospital of Jilin University.

Population: HBsAg-positive and hepatitis B e antigen (HBeAg) -positive pregnant women were recruited.

Methods: Blood samples were collected from mothers before delivery, and HBV infection of infants was evaluated at 7 months of age.

Results: Overall, 268 mothers and 271 infants were enrolled. HBV DNA and HBsAg levels were correlated (rs = 0.699; P < 0.001), and HBV DNA (rs = 0.500; P < 0.001) and HBsAg (rs = 0.372; P < 0.001) were both correlated with HBV RNA. The areas under the curve for HBV DNA, HBsAg and HBV RNA for prediction of infection were 0.69 (95% CI 0.57-0.82), 0.63 (95% CI 0.51-0.76) and 0.65 (95% CI 0.52-0.78), respectively. Higher HBV DNA (odds ratio [OR] 4.77, 95% CI 1.44-15.86), higher HBsAg (OR 4.13, 95% CI 1.12-15.25) and higher HBV RNA (OR 3.19, 95% CI 1.09-9.32) were risk factors for HBV infection. Analysis of the HBV DNA-RNA-HBsAg Score revealed that it was an independent predictive factor for mother-to-child transmission (the OR of Score 3 was 8.81, 95% CI 2.79-27.82).

Conclusion: HBV DNA, HBV RNA and HBsAg were correlated in HBeAg-positive pregnant women. HBsAg could be considered as a substitute marker of HBV DNA for HBeAg-positive pregnant women in low-income regions. We should pay special attention to pregnant women with high levels of all three markers.

Tweetable abstract: HBsAg could be considered as a substitute marker of HBV DNA for HBeAg-positive pregnant women in low-income regions. Special attention should be given to pregnant women with high levels of all three markers (HBV DNA, HBV RNA and HBsAg).

Keywords: hepatitis B e antigen; hepatitis B surface antigen; hepatitis B virus DNA; hepatitis B virus RNA; mother-to-child-transmission; pregnant women.

Publication types

  • Observational Study

MeSH terms

  • Adult
  • Cohort Studies
  • DNA, Viral
  • Female
  • Hepatitis B / blood
  • Hepatitis B / transmission*
  • Hepatitis B Surface Antigens / blood
  • Hepatitis B virus / genetics
  • Hepatitis B virus / immunology
  • Hepatitis B virus / isolation & purification*
  • Humans
  • Infant
  • Infant, Newborn
  • Infectious Disease Transmission, Vertical*
  • Male
  • Predictive Value of Tests
  • Pregnancy
  • Pregnancy Complications, Infectious / virology*
  • Prospective Studies
  • RNA, Viral
  • Retrospective Studies
  • Viral Load

Substances

  • DNA, Viral
  • Hepatitis B Surface Antigens
  • RNA, Viral