Delayed positive COVID19 nasopharyngeal test, a case study with clinical and pathological correlation

BMC Pulm Med. 2021 Aug 31;21(1):278. doi: 10.1186/s12890-021-01643-y.

Abstract

Background: There are various reasons for delayed positive nasopharyngeal PCR tests for coronavirus disease 2019 (COVID19) in not only asymptomatic but also severely diseased patients. The pathophysiological attributes are not known. We explore this possibility through a case report.

Case presentation: A 64-year-old male with history of pulmonary fungal infection, asthma and chronic pulmonary obstructive disease (COPD), diabetes, coronary artery disease presented with shortness of breath, fever and chest image of ground opacity, reticular interstitial thickening, highly suspicious for COVID19. However, nasopharyngeal swab tests were discordantly negative for four times in two weeks, and IgG antibody for COVID19 was also negative. However, serum IgE level was elevated. No other pathogens are identified. His symptoms deteriorated despite corticosteroid, antibiotics and bronchodilator treatment. Bronchoalveolar lavage (BAL) and open lung wedge biopsy were performed for etiology diagnosis. They demonstrated COVID19 viral RNA positive fibrosing organizing pneumonia with respiratory tract damage characterized by suspicious viral cytopathic effect, mixed neutrophilic, lymphoplasmacytic, histiocytic and eosinophilic inflammation and fibrosis besides expected asthma and COPD change. One week later, repeated COVID19 nasopharyngeal tests on day 40 and day 49 became positive.

Conclusion: Our case and literature review indicate that allergic asthma and associated high IgE level together with corticosteroid inhalation might contribute to the delayed positive nasopharyngeal swab in upper airway; COPD related chronic airways obstruction and the addition of fibrosis induced ventilator dependence and poor prognosis in COVID19 pneumonia, and should be therapeutically targeted besides antiviral therapy.

Keywords: Asthma; BAL; COPD; COVID19; Corticosteroid; Nasopharyngeal swab.

Publication types

  • Case Reports

MeSH terms

  • Administration, Inhalation
  • Adrenal Cortex Hormones / therapeutic use
  • Asthma / complications
  • Asthma / drug therapy
  • Asthma / pathology
  • Bronchoalveolar Lavage
  • COVID-19 / complications
  • COVID-19 / diagnosis*
  • COVID-19 / therapy
  • COVID-19 Nucleic Acid Testing*
  • Delayed Diagnosis*
  • Fatal Outcome
  • Humans
  • Male
  • Middle Aged
  • Nasopharynx / virology*
  • Pulmonary Disease, Chronic Obstructive / complications
  • Pulmonary Disease, Chronic Obstructive / drug therapy
  • Pulmonary Disease, Chronic Obstructive / pathology
  • Reverse Transcriptase Polymerase Chain Reaction
  • SARS-CoV-2 / isolation & purification*

Substances

  • Adrenal Cortex Hormones