Secondary Structure Analysis of Single Silk Nanofibril through Infrared Nanospectroscopy

Yawen Liu; Hongchong Guo; Shengjie Ling

doi:10.1007/978-1-0716-1574-4_19

Secondary Structure Analysis of Single Silk Nanofibril through Infrared Nanospectroscopy

Methods Mol Biol. 2021:2347:221-228. doi: 10.1007/978-1-0716-1574-4_19.

Authors

Yawen Liu¹, Hongchong Guo¹, Shengjie Ling²

Affiliations

¹ School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.
² School of Physical Science and Technology, ShanghaiTech University, Shanghai, China. [email protected].

PMID: 34472068
DOI: 10.1007/978-1-0716-1574-4_19

Abstract

Infrared nanospectroscopy (NanoIR) is a new experimental technique to research the secondary structure of protein-based nanoarchitectures in recent years. Compared with the conventional IR, NanoIR reveals to be an exquisite, sensitive, and accurate tool to analyze and image the single molecule secondary structure, which can reach up to high spatial resolution (10 nm). Here we present a detailed protocol to introduce how to study single silk nanofibril (SNF) and process the results by this routine. This protocol provides a useful method to demonstrate the microstructure of nanomaterials by NanoIR, displaying the potential application in analytical chemistry, biomaterials, and nanotechnologies.

Keywords: Infrared nanospectroscopy; Nanomaterials; Second structure; Silk nanofibril.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Nanostructures*
Nanotechnology
Protein Structure, Secondary
Silk*
Spectrophotometry, Infrared

Substances

Silk