A BOSSS method for managing insights into diet-microbiome interactions

Gut microbial metabolites generated through interactions with dietary constituents are important modulators of host metabolism and physiology. Our understanding of diet-microbiome-host interactions is hampered by the limited tools to broadly identify microbial species that take up nutrients and their products. BioOrthogonal labeling-Sort-Sequence-Spectrometry (BOSSS) overcomes these challenges by leveraging orally introduced alkyne-modified lipids that permit identification of gut microbes that take up dietary lipids.