Novel enzymatic graphene oxide based biosensor for the detection of glutathione in biological body fluids

Somaye Cheraghi; Mohammad A Taher; H Karimi-Maleh; Fatmeh Karimi; Mehdi Shabani-Nooshabadi; Marzieh Alizadeh; Amani Al-Othman; Nevin Erk; Praveen Kumar Yegya Raman; Ceren Karaman

doi:10.1016/j.chemosphere.2021.132187

Novel enzymatic graphene oxide based biosensor for the detection of glutathione in biological body fluids

Chemosphere. 2022 Jan;287(Pt 2):132187. doi: 10.1016/j.chemosphere.2021.132187. Epub 2021 Sep 7.

Authors

Affiliations

¹ Iran National Science Foundation (INSF), Tehran, Iran; Department of Chemistry, Shahid Bahonar University of Kerman, Iran. Electronic address: [email protected].
² Department of Chemistry, Shahid Bahonar University of Kerman, Iran. Electronic address: [email protected].
³ Department of Chemical Engineering and Energy, Quchan University of Technology, Quchan, 9477177870, Iran. Electronic address: [email protected].
⁴ Department of Chemical Engineering and Energy, Quchan University of Technology, Quchan, 9477177870, Iran.
⁵ Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan, Islamic Republic of Iran.
⁶ Laboratory of Basic Sciences, Mohammad Rasul Allah Research Tower, Shiraz University of Medical Sciences, Shiraz, Iran.
⁷ Department of Chemical Engineering, American University of Sharjah, Sharjah, PO. Box 26666, United Arab Emirates.
⁸ Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, 06560, Ankara, Turkey.
⁹ Textile Department, North Carolina State University, USA.
¹⁰ Akdeniz University, Vocational School of Technical Sciences, Department of Electricity and Energy, Antalya, Turkey. Electronic address: [email protected].

PMID: 34509007
DOI: 10.1016/j.chemosphere.2021.132187

Abstract

In this work, we report a novel enzymatic biosensor based on glutathione peroxidase (GSH-Px), graphene oxide (GO) and nafion for the electrochemical sensing of glutathione (GSH) in body fluids. GSH-Px was immobilized covalently via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) onto modified glassy carbon electrode (GCE) decorated with GO and nafion and successfully used for sensing of GSH in the presence of H₂O₂ as catalyst with Michaelis-Menten constant about 0.131 mmol/L. The active surface are of GCE improve from 0.183 cm² to 0.225 cm² after modification with GO. The introduced biosensor (GSH-Px/GO/nafion/GCE) was used for monitoring of GSH over the range 0.003-370.0 μM, with a detection limit of 1.5 nM using differential pulse voltammetric (DPV) method. The GSH-Px/GO/nafion/GCE was successfully applied to the determination of GSH in real samples.

Keywords: Enzymatic biosensor; Glutathione; Glutathione peroxidase; Graphene oxide; Nafion; Voltammetric method.

MeSH terms

Biosensing Techniques*
Body Fluids*
Glutathione
Graphite
Hydrogen Peroxide

Substances

graphene oxide
Graphite
Hydrogen Peroxide
Glutathione