The glycine arginine-rich domain of the RNA-binding protein nucleolin regulates its subcellular localization

EMBO J. 2021 Oct 18;40(20):e107158. doi: 10.15252/embj.2020107158. Epub 2021 Sep 13.

Abstract

Nucleolin is a multifunctional RNA Binding Protein (RBP) with diverse subcellular localizations, including the nucleolus in all eukaryotic cells, the plasma membrane in tumor cells, and the axon in neurons. Here we show that the glycine arginine rich (GAR) domain of nucleolin drives subcellular localization via protein-protein interactions with a kinesin light chain. In addition, GAR sequences mediate plasma membrane interactions of nucleolin. Both these modalities are in addition to the already reported involvement of the GAR domain in liquid-liquid phase separation in the nucleolus. Nucleolin transport to axons requires the GAR domain, and heterozygous GAR deletion mice reveal reduced axonal localization of nucleolin cargo mRNAs and enhanced sensory neuron growth. Thus, the GAR domain governs axonal transport of a growth controlling RNA-RBP complex in neurons, and is a versatile localization determinant for different subcellular compartments. Localization determination by GAR domains may explain why GAR mutants in diverse RBPs are associated with neurodegenerative disease.

Keywords: axonal transport; cell size regulation; local translation; protein-membrane interaction; subcellular localization.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Axonal Transport / genetics
  • Cell Line, Tumor
  • Cell Nucleolus / metabolism*
  • Cell Nucleolus / ultrastructure
  • Ganglia, Spinal / cytology
  • Ganglia, Spinal / metabolism*
  • Gene Expression
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Kinesins / genetics
  • Kinesins / metabolism*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mutation
  • Neurons / cytology
  • Neurons / metabolism*
  • Nucleolin
  • Phosphoproteins / chemistry*
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism
  • Primary Cell Culture
  • Protein Domains
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA-Binding Proteins / chemistry*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism
  • Sciatic Nerve / cytology
  • Sciatic Nerve / metabolism*

Substances

  • Kns2 protein, mouse
  • Phosphoproteins
  • RNA, Messenger
  • RNA-Binding Proteins
  • Kinesins