Isolation and Detection of Murine iNKT Cells in Different Organs

Mengqing Cong; Xiang Li; Haopeng Fang; Li Bai; Xucai Zheng; Bofeng Li

doi:10.1007/978-1-0716-1775-5_5

Isolation and Detection of Murine iNKT Cells in Different Organs

Methods Mol Biol. 2021:2388:59-69. doi: 10.1007/978-1-0716-1775-5_5.

Authors

Mengqing Cong^#^{1

2}, Xiang Li^#², Haopeng Fang^{1

2}, Li Bai², Xucai Zheng³, Bofeng Li^{4

5}

Affiliations

¹ Division of Life Sciences and Medicine, Department of Medical Oncology, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, China.
² Hefei National Laboratory for Physical Sciences at Microscale, Laboratory of Structural Immunology, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
³ Division of Life Sciences and Medicine, Department of Medical Oncology, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, China. [email protected].
⁴ Division of Life Sciences and Medicine, Department of Medical Oncology, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, China. [email protected].
⁵ Hefei National Laboratory for Physical Sciences at Microscale, Laboratory of Structural Immunology, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China. [email protected].

^# Contributed equally.

PMID: 34524661
DOI: 10.1007/978-1-0716-1775-5_5

Abstract

The invariant NKT (iNKT) cells are innate-like lymphocytes that share phenotypic and functional characteristics with NK cells and T cells, playing an important role in both human and mouse physiology and disease and bridging the gap between the innate and adaptive immune responses. The frequency and subtypes of iNKT cells in major immune organs are different, which also determines the regional immune characteristics of iNKT cells. Here, we report a protocol about the isolation of iNKT cells in the thymus, spleen, and liver of C57BL/6, CD1d^-/-, and Jα18^-/- mice.

Keywords: Liver; Spleen; Thymus; iNKT cells.

MeSH terms

Animals
Antigens, CD1d / genetics
Cell Separation
Mice
Mice, Inbred C57BL
Natural Killer T-Cells*
Spleen

Substances

Antigens, CD1d