Modeling Human Genetic Disorders with CRISPR Technologies in Xenopus

Helen Rankin Willsey; Matthew Guille; Robert M Grainger

doi:10.1101/pdb.prot106997

Modeling Human Genetic Disorders with CRISPR Technologies in Xenopus

Cold Spring Harb Protoc. 2022 Mar 1;2022(3). doi: 10.1101/pdb.prot106997.

Authors

Helen Rankin Willsey¹, Matthew Guille², Robert M Grainger³

Affiliations

¹ Department of Psychiatry and Behavioral Sciences, Weill Institute for Neurosciences, University of California, San Francisco, San Francisco, California 94143, USA; [email protected] [email protected].
² European Xenopus Resource Centre, School of Biological Sciences, University of Portsmouth, Portsmouth PO1 2UP, United Kingdom.
³ Department of Biology, University of Virginia, Charlottesville, Virginia 22904, USA [email protected] [email protected].

PMID: 34531330
DOI: 10.1101/pdb.prot106997

Abstract

Combining the power of Xenopus developmental biology with CRISPR-based technologies promises great discoveries in understanding and treating human genetic disorders. Here we provide a practical pipeline for how to go from known disease gene(s) or risk gene(s) of interest to methods for gaining functional insight into the contribution of these genes to disorder etiology in humans.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
CRISPR-Cas Systems* / genetics
Gene Editing* / methods
Humans
Xenopus laevis / genetics

Abstract

Publication types

MeSH terms

Grants and funding