Surface plasmon resonance approach to study drug interactions with SARS-CoV-2 RNA-dependent RNA polymerase highlights treatment potential of suramin

Martina Mravinec; Gregor Bajc; Matej Butala

doi:10.1016/j.jviromet.2021.114283

Surface plasmon resonance approach to study drug interactions with SARS-CoV-2 RNA-dependent RNA polymerase highlights treatment potential of suramin

J Virol Methods. 2021 Dec:298:114283. doi: 10.1016/j.jviromet.2021.114283. Epub 2021 Sep 14.

Authors

Martina Mravinec¹, Gregor Bajc¹, Matej Butala²

Affiliations

¹ Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana 1000, Slovenia.
² Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana 1000, Slovenia. Electronic address: [email protected].

Abstract

The SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) is essential for virus replication, therefore it is a promising drug target. Here we present a surface plasmon resonance approach to study the interaction of RdRp with drugs in real time. We monitored the effect of favipiravir, ribavirin, sofosbuvir triphosphate PSI-7409 and suramin on RdRp binding to RNA immobilized on the chip. Suramin precluded interaction of RdRp with RNA and even displaced RdRp from RNA.

Keywords: RNA-dependent RNA polymerase; SARS-CoV-2; Suramin; Surface plasmon resonance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antiviral Agents / pharmacology
COVID-19*
Drug Interactions
Humans
RNA, Viral
RNA-Dependent RNA Polymerase*
SARS-CoV-2
Suramin / pharmacology
Surface Plasmon Resonance

Substances

Antiviral Agents
RNA, Viral
Suramin
RNA-Dependent RNA Polymerase