Organic mercury solid phase chemoselective capture for proteomic identification of S-nitrosated proteins and peptides

Nitric Oxide. 2021 Dec 1:117:1-6. doi: 10.1016/j.niox.2021.09.004. Epub 2021 Sep 15.

Abstract

Cysteine S-nitrosation mediates NO signaling and protein function under pathophysiological conditions. Herein, we provide a detailed protocol regarding the organic mercury chemoselective enrichment of S-nitrosated proteins and peptides. We discuss key aspects of the enrichment strategy and provide technical tips for the best performance of the experimental protocol.

Keywords: Cysteine S-Nitrosation; Enrichment strategy; Nitric oxide signaling; Organic mercury; Protein; Proteomics.

MeSH terms

  • Chromatography
  • Cysteine / analysis
  • Cysteine / isolation & purification
  • Cysteine / metabolism
  • Mercury / chemistry*
  • Nitrates* / analysis
  • Nitrates* / isolation & purification
  • Nitrates* / metabolism
  • Nitric Oxide / metabolism
  • Nitrosation
  • Peptides / analysis
  • Peptides / isolation & purification
  • Peptides / metabolism
  • Proteins* / analysis
  • Proteins* / isolation & purification
  • Proteins* / metabolism
  • Proteomics / methods*

Substances

  • Nitrates
  • Peptides
  • Proteins
  • Nitric Oxide
  • Mercury
  • Cysteine