Green Approach Towards Morphology-Controlled Synthesis of Zein-Functionalized TiO₂ Nanoparticles for Cosmeceutical Application

Biomolecular approaches for synthesis of inorganic nanoparticle are very popular among researchers and exhibit significant shape-directing morphologies in classified condition. The proteins are the most abundant macromolecules and employed for the hybrid synthesis as well as shape-directing agent. The present study is designed to investigate the potential role of a plant protein 'zein' to synthesize hybrid TiO₂ nanoparticles. This versatile amphiphilic protein paves a unique path towards shape directing synthesis and act as template in the biomineralization process. The structural changes occurred in protein structure is thoroughly characterized using the circular dichroism (CD) and FTIR spectroscopy. UV, XPS and HRTEM analysis confirms the presence of zein on the nanoparticle surface. The proposed approach provides finely engineered nano-cuboidal (22.75±5.07 nm) geometry with homogenous dispersion, curved edged cuboids (403.51±0.05 nm) and spherical (97.85±0.62 nm) shaped from different modification, as evidenced by TEM. We also discussed in-vitro method for the detection of antimicrobial activity of nanocuboids against acne causing microorganisms such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Streptococcus agalactiae. Our results demonstrate that hybrid nanocuboids could be an efficient green material and provide cognitive antimicrobial evidence that could be deployed for cosmeceutical application.