RNA Strand Displacement Assay for Hepatitis E Virus Helicase

Vidya P Nair; Milan Surjit

doi:10.21769/BioProtoc.2198

RNA Strand Displacement Assay for Hepatitis E Virus Helicase

Bio Protoc. 2017 Apr 5;7(7):e2198. doi: 10.21769/BioProtoc.2198.

Authors

Vidya P Nair¹, Milan Surjit¹

Affiliation

¹ Virology Laboratory, Vaccine and Infectious Disease Research Centre, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, Faridabad, Haryana, India.

Abstract

The hepatitis E virus (HEV) helicase uses ATP to unwind the RNA duplexes. This is an essential step for viral replication. This protocol aims to measure the double strand RNA unwinding activity of the HEV helicase.

Keywords: Helicase; Helicase assay; Hepatitis E virus; Nonradioactive helicase assay; RNA virus; Viral replication.