The aim of this study was to understand the research capacity and culture of a regional allied health workforce over time. A cross-sectional study design was used, with data collected using the validated Research Capacity and Culture (RCC) tool. The results were compared with an earlier administration of the RCC survey. The findings demonstrate that allied health professionals (AHPs) perceive that the organisation's capability of conducting research is at a higher level than that of teams and individuals. Over a 4-year period the profile of the research culture of an allied health workforce in a regional health service was described similarly. The highest rated motivator for conducting research (to develop skills) and barrier to conducting research (other work roles take priority) were unchanged between 2018 and 2014. AHPs have maintained the previous viewpoint that there is research capacity at the health service and opportunities to develop the research culture. The findings of the 2018 data compared with the 2014 data highlight that specific and targeted research capacity-building strategies need to be used in order to create an active and vibrant research culture.