The complete chloroplast genome of Lonicera pampaninii Levl. and its phylogenetic analysis

Mitochondrial DNA B Resour. 2021 Sep 22;6(10):3025-3027. doi: 10.1080/23802359.2021.1978891. eCollection 2021.

Abstract

Lonicera pampaninii Levl, a Chinese herbal medicine widely used in the folk, has the effect of clearing away heat and detoxifying similar to other plants of the Lonicera. However, its genetic relationship with these plants is unclear. In this work, the cp genome of Lonicera pampaninii Levl. was assembled by the high-throughput Illumina pair-end sequencing data. The circular cp genome is 155,249 bp in size, including a large single-copy (LSC) region of 89,068 bp and a small single-copy (SSC) region of 18,635 bp, which were separated by two inverted repeat (IR) regions (23,773 bp each). A total of 120 genes were predicted, including eight ribosomal RNAs (rRNAs), 33 transfer RNAs (tRNAs), and 79 protein-coding genes (PCGs). Furthermore, phylogenetic analysis revealed a strong sister relationship between L. pampaninii and other two congeneric species (Lonicera confusa and Lonicera japonica). This study provides useful information for future genetic study of L. pampaninii.

Keywords: Caprifoliaceae; Lonicera pampaninii; complete chloroplast genome; phylogenetic.

Grants and funding

This study was supported by the National Natural Science Foundation of China under Grant [Nos. U1812401 and 32060068].