Telomere length assessments of muscle stem cells in rodent and human skeletal muscle sections

Elisia D Tichy; Foteini Mourkioti

doi:10.1016/j.xpro.2021.100830

Telomere length assessments of muscle stem cells in rodent and human skeletal muscle sections

STAR Protoc. 2021 Sep 14;2(4):100830. doi: 10.1016/j.xpro.2021.100830. eCollection 2021 Dec 17.

Authors

Elisia D Tichy¹, Foteini Mourkioti^{1

2

3}

Affiliations

¹ Department of Orthopaedic Surgery, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA 19104, USA.
² Department of Cell and Developmental Biology, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA 19104, USA.
³ Institute of Regenerative Medicine, Musculoskeletal Regeneration Program, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA 19104, USA.

Abstract

Measurements of telomere length in skeletal muscle stem cells (MuSCs), a rare cell population within muscles, provide insights into cellular dysfunction in diseased conditions. Here, we describe a protocol (cryosection muscle quantitative fluorescent in situhybridization) using skeletal muscle cryosections for assessments of telomere length in MuSCs, in their native environment. Using a free software, telomere length measurements are assessed on a single-cell level. We also provide methodology to perform data analyses in several ways. For complete details on the use and execution of this protocol, please refer to Tichy et al. (2021).

Keywords: Cell Biology; Cell isolation; Cell-based Assays; In Situ Hybridization; Microscopy; Stem Cells.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Humans
Muscle Fibers, Skeletal
Muscle, Skeletal
Myoblasts*
Rodentia*
Telomere / genetics

Abstract

Publication types

MeSH terms

Grants and funding