Isolation of mouse Kupffer cells for phenotypic and functional studies

STAR Protoc. 2021 Sep 14;2(4):100831. doi: 10.1016/j.xpro.2021.100831. eCollection 2021 Dec 17.

Abstract

Here, we provide detailed protocols for the isolation of mouse Kupffer cells - the liver-resident macrophages - for phenotypic (e.g., via flow cytometry, mass cytometry, or RNA-sequencing) analyses or for functional experiments involving cell culture. The procedures presented can be adapted for the isolation of other hepatic cell populations. For complete details on the use and execution of this protocol, please refer to De Simone et al. (2021).

Keywords: Cell culture; Cell isolation; Flow Cytometry/Mass Cytometry; Immunology; Single Cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques*
  • Flow Cytometry / methods
  • Kupffer Cells*
  • Mice