Novel prognostic model established for patients with head and neck squamous cell carcinoma based on pyroptosis-related genes

Yuchen Shen; Xinyu Li; Deming Wang; Liming Zhang; Xiao Li; Tong Xia; Xunjie Shang; Xitao Yang; Lixin Su; Xindong Fan

doi:10.1016/j.tranon.2021.101233

Novel prognostic model established for patients with head and neck squamous cell carcinoma based on pyroptosis-related genes

Transl Oncol. 2021 Dec;14(12):101233. doi: 10.1016/j.tranon.2021.101233. Epub 2021 Sep 29.

Authors

Yuchen Shen¹, Xinyu Li², Deming Wang¹, Liming Zhang¹, Xiao Li¹, Tong Xia¹, Xunjie Shang¹, Xitao Yang³, Lixin Su⁴, Xindong Fan⁵

Affiliations

¹ Department of Interventional Therapy, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Centre for Oral Diseases, Shanghai, China.
² Department of Neurosurgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No.280 Mo-He Road, Shanghai, China.
³ Department of Interventional Therapy, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Centre for Oral Diseases, Shanghai, China. Electronic address: [email protected].
⁴ Department of Interventional Therapy, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Centre for Oral Diseases, Shanghai, China. Electronic address: [email protected].
⁵ Department of Interventional Therapy, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Centre for Oral Diseases, Shanghai, China. Electronic address: [email protected].

Abstract

We aimed at establishing a risk - score model using pyroptosis-related genes to predict the prognosis of patients with head and neck squamous cell carcinoma (HNSCC). A total of 33 pyroptosis-related genes were selected. We then evaluated the data of 502 HNSCC patients and 44 normal patients from TCGA database. Gene expression was then profiled to detect differentially expressed genes (DEGs). Using the univariate, the least absolute shrinkage and selection operator (LASSO) Cox regression analyses, we generated a risk - score model. Tissue samples from neoplastic and normal sites of 44 HNSCC patients were collected. qRT-PCR were employed to analyze the mRNA level of the samples. Kaplan-Meier method was used to evaluate the overall survival rate (OS). Enrichment analysis was performed to elucidate the underlying mechanism of HNSCC patient's differentially survival status from the perspective of tumor immunology. 17 genes were categorized as DEGs. GSDME, IL-6, CASP8, CASP6, NLRP1 and NLRP6 were used to establish the risk - score model. Each patient's risk score in the TCGA cohort was calculated using the risk - score formula. The risk score was able to independently predict the OS of the HNSCC patients (P = 0.02). The OS analysis showed that the risk score model (P < 0.0001) was more reliable than single gene, a phenomenon verified by practical patient cohort. Additionally, enrichment analysis indicated more active immune activities in low-risk group than high-risk group. In conclusion, our risk - score model has provided novel strategy for the prediction of HNSCC patients' prognosis.

Keywords: Carcinoma; HNSCC; Prediction; Prognosis; Pyroptosis.