Abstract
Newborn screening using dried plasma spots offers preanalytical advantages over conventional cards for plasma-associated targets of interest. Herein we present dried plasma spot-based methods for measuring metabolites using a 250+ compound liquid chromatography tandem mass spectrometry library. Quality assurance reduced this library to 134, and from these, 30 compounds determined the normal newborn reference ranges.
Copyright © 2021 Elsevier Inc. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Biomarkers / blood*
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Blood Preservation / methods
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Blood Preservation / standards
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Chromatography, Liquid*
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Dried Blood Spot Testing / methods*
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Dried Blood Spot Testing / standards
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Female
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Humans
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Infant
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Infant, Newborn
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Male
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Metabolome*
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Neonatal Screening / methods*
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Neonatal Screening / standards
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Prospective Studies
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Reference Values
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Specimen Handling / methods
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Specimen Handling / standards
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Tandem Mass Spectrometry*