Arabidopsis Growth and Dissection on Polyethersulfone (PES) Membranes for Gravitropic Studies

Alexander Meyers; Nathan Scinto-Madonich; Sarah E Wyatt; Chris Wolverton

doi:10.1007/978-1-0716-1677-2_15

Arabidopsis Growth and Dissection on Polyethersulfone (PES) Membranes for Gravitropic Studies

Methods Mol Biol. 2022:2368:233-239. doi: 10.1007/978-1-0716-1677-2_15.

Authors

Alexander Meyers^{1

2}, Nathan Scinto-Madonich³, Sarah E Wyatt^{1

2}, Chris Wolverton⁴

Affiliations

¹ Department of Environmental & Plant Biology, Ohio University, Athens, OH, USA.
² Program in Molecular and Cellular Biology. Ohio University, Athens, OH, USA.
³ Graduate Field of Plant Biology. Cornell University, Ithaca, NY, USA.
⁴ Department of Botany and Microbiology, Ohio Wesleyan University, Delaware, OH, USA. [email protected].

PMID: 34647259
DOI: 10.1007/978-1-0716-1677-2_15

Abstract

Polyethersulfone (PES) membranes provide a versatile tool for gravity-related plant studies. Benefits of this system include straightforward setup, no need for specialized equipment, long-term seed viability between plating and hydration/growth, and adaptability to diverse protocols and downstream analyses. Methods outlined here include seed sterilization, planting, growth, and dissection that will transition directly into any RNA extraction protocol.

Keywords: Gravitropism; Polyethersulfone; Spaceflight.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Arabidopsis Proteins
Arabidopsis*
Dissection
Gravitropism
Plant Roots
Polymers
Sulfones

Substances

Arabidopsis Proteins
Polymers
Sulfones
polyether sulfone