Super-resolved Raman microscopy using random structured light illumination: Concept and feasibility

Hongki Lee; Hajun Yoo; Gwiyeong Moon; Kar-Ann Toh; Kentaro Mochizuki; Katsumasa Fujita; Donghyun Kim

doi:10.1063/5.0064082

Super-resolved Raman microscopy using random structured light illumination: Concept and feasibility

J Chem Phys. 2021 Oct 14;155(14):144202. doi: 10.1063/5.0064082.

Authors

Hongki Lee¹, Hajun Yoo¹, Gwiyeong Moon¹, Kar-Ann Toh¹, Kentaro Mochizuki², Katsumasa Fujita³, Donghyun Kim¹

Affiliations

¹ School of Electrical and Electronic Engineering, Yonsei University, Seoul 03722, South Korea.
² Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
³ Department of Applied Physics, Osaka University, Osaka 565-0871, Japan and Advanced Photonics and Biosensing Open Innovation Laboratory, AIST-Osaka University, Osaka 565-0871, Japan.

PMID: 34654313
DOI: 10.1063/5.0064082

Abstract

In this article, we report the use of randomly structured light illumination for chemical imaging of molecular distribution based on Raman microscopy with improved image resolution. Random structured basis images generated from temporal and spectral characteristics of the measured Raman signatures were superposed to perform structured illumination microscopy (SIM) with the blind-SIM algorithm. For experimental validation, Raman signatures corresponding to Rhodamine 6G (R6G) in the waveband of 730-760 nm and Raman shift in the range of 1096-1634 cm^-1 were extracted and reconstructed to build images of R6G. The results confirm improved image resolution using the concept and hints at super-resolution by almost twice better than the diffraction-limit.