PD-L1 immunostaining: what pathologists need to know

Diagn Pathol. 2021 Oct 25;16(1):94. doi: 10.1186/s13000-021-01151-x.

Abstract

Background: Immune checkpoint proteins, especially PD-L1 and PD-1, play a crucial role in controlling the intensity and duration of the immune response, thus preventing the development of autoimmunity. These proteins play a vital role in enabling cancer cells to escape immunity, proliferate and progress.

Methods: This brief review highlights essential points related to testing for immune checkpoint therapy that histopathologists need to know.

Results: In recent years, several inhibitors of these proteins have been used to reactivate the immune system to fight cancer. Selection of patients for such therapy requires demonstration of PD-L1 activation on the tumor cells, best done by immunohistochemical staining of the tumor and immune cells using various antibodies with predetermined thresholds.

Conclusions: Immune checkpoint therapy appears to be promising and is rapidly expanding to include a large variety of cancers.

Keywords: Activation; Cancer; Immune cells; Immune checkpoint; Immunohistochemistry; Inhibition; Inhibitors; PD-1; PD-L1; T-cells.

Publication types

  • Review

MeSH terms

  • B7-H1 Antigen / analysis*
  • B7-H1 Antigen / antagonists & inhibitors
  • Biomarkers, Tumor / analysis*
  • Biomarkers, Tumor / antagonists & inhibitors
  • Clinical Decision-Making
  • Humans
  • Immune Checkpoint Inhibitors / therapeutic use
  • Immunohistochemistry*
  • Neoplasms / chemistry*
  • Neoplasms / drug therapy
  • Neoplasms / pathology
  • Pathologists*
  • Predictive Value of Tests
  • Programmed Cell Death 1 Receptor / analysis*
  • Programmed Cell Death 1 Receptor / antagonists & inhibitors
  • Reproducibility of Results

Substances

  • B7-H1 Antigen
  • Biomarkers, Tumor
  • CD274 protein, human
  • Immune Checkpoint Inhibitors
  • PDCD1 protein, human
  • Programmed Cell Death 1 Receptor