The complete mitochondrial genome for Stanley's slug snake Pareas stanleyi (Serpentes: Pareidae) by next-generation sequencing

Xian Yang; Yu-Qian Shen; Jun-Jie Zhong; Li Wei; Zhi-Hua Lin; Li Ma

doi:10.1080/23802359.2021.1920508

The complete mitochondrial genome for Stanley's slug snake Pareas stanleyi (Serpentes: Pareidae) by next-generation sequencing

Mitochondrial DNA B Resour. 2021 Oct 23;6(11):3265-3266. doi: 10.1080/23802359.2021.1920508. eCollection 2021.

Authors

Xian Yang¹, Yu-Qian Shen¹, Jun-Jie Zhong¹, Li Wei¹, Zhi-Hua Lin¹, Li Ma¹

Affiliation

¹ Laboratory of Amphibian Diversity Investigation, College of Ecology, Lishui University, Lishui, Zhejiang, China.

Abstract

The complete mitochondrial DNA (mtDNA) for Pareas stanleyi was determined in this study. The length of complete mtDNA was 18,932 bp, including 13 protein-coding genes (PCGs) (COI-III, ND1-6, ND4L, ATP6, ATP8 and CYTB), 23 tRNA genes, 2 rRNA genes, a L-chain replication-initiating non-coding region (NCR) and 2 control regions. The overall base composition of the sequence is 24.76% of T, 29.20% of C, 30.87% of A, and 15.16% of G, with a total A + T content of 55.63%. The phylogenetic tree showed that P. stanleyi had a close relationship with the other two species (P. boulengeri and P. formosensis) from the genus Pareas.

Keywords: Pareas stanleyi; mitochondrial DNA; next-generation sequencing; phylogenetic tree.

Grants and funding

This work was supported by Zhejiang Provincial Natural Science Foundation of China [LQ20C030001], National Natural Science Foundation of China [32001243], and Public Welfare Technology Application Research Project of Lishui City [2020GYX06].