PAD4 controls chemoattractant production and neutrophil trafficking in malaria

J Leukoc Biol. 2022 Jun;111(6):1235-1242. doi: 10.1002/JLB.4AB1120-780R. Epub 2021 Nov 10.

Abstract

Peptidylarginine deiminase 4 (PAD4) is a key regulator of inflammation but its function in infections remains incompletely understood. We investigate PAD4 in the context of malaria and demonstrate a role in regulation of immune cell trafficking and chemokine production. PAD4 regulates liver immunopathology by promoting neutrophil trafficking in a Plasmodium chabaudi mouse malaria model. In human macrophages, PAD4 regulates expression of CXCL chemokines in response to stimulation with TLR ligands and P. falciparum. Using patient samples, we show that CXCL1 may be a biomarker for severe malaria. PAD4 inhibition promotes disease tolerance and may represent a therapeutic avenue in malaria.

Keywords: PAD4; chemokine; malaria; neutrophil.

MeSH terms

  • Animals
  • Chemotactic Factors
  • Disease Models, Animal
  • Humans
  • Malaria* / metabolism
  • Mice
  • Neutrophils*
  • Protein-Arginine Deiminase Type 4

Substances

  • Chemotactic Factors
  • Protein-Arginine Deiminase Type 4