Towards inferring nanopore sequencing ionic currents from nucleotide chemical structures

Nat Commun. 2021 Nov 11;12(1):6545. doi: 10.1038/s41467-021-26929-x.

Abstract

The characteristic ionic currents of nucleotide kmers are commonly used in analyzing nanopore sequencing readouts. We present a graph convolutional network-based deep learning framework for predicting kmer characteristic ionic currents from corresponding chemical structures. We show such a framework can generalize the chemical information of the 5-methyl group from thymine to cytosine by correctly predicting 5-methylcytosine-containing DNA 6mers, thus shedding light on the de novo detection of nucleotide modifications.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cytosine / metabolism
  • Nanopore Sequencing / methods
  • Nucleotides / metabolism*
  • Sequence Analysis, DNA / methods

Substances

  • Nucleotides
  • Cytosine