Indirect Immunofluorescence of Tissue Sections

Cody J Aros

doi:10.1007/978-1-0716-1771-7_2

Indirect Immunofluorescence of Tissue Sections

Methods Mol Biol. 2022:2386:17-26. doi: 10.1007/978-1-0716-1771-7_2.

Author

Cody J Aros^{1

2

3}

Affiliations

¹ UCLA Department of Molecular Biology Interdepartmental Program, UCLA, Los Angeles, CA, USA. [email protected].
² UCLA Medical Scientist Training Program, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA. [email protected].
³ UCLA Children's Discovery and Innovation Institute, Mattel Children's Hospital UCLA, Department of Pediatrics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA. [email protected].

PMID: 34766262
DOI: 10.1007/978-1-0716-1771-7_2

Abstract

Immunofluorescence (IF) on tissue sections allows for the detection of protein species subcellular localization. IF studies further offer the ability to achieve this understanding at the level of single cell granularity. Here, we describe the processes by which tissue is fixed, embedded, sectioned, and subsequently utilized for conducting indirect IF assays. We raise potential opportunities for troubleshooting and optimization at varying stages of the protocol.

Keywords: Antibody; Antigen retrieval; Blocking; Immunofluorescence; Permeabilization.

MeSH terms

Fluorescent Antibody Technique, Indirect*
Staining and Labeling
Tissue Fixation