Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds

Mitchell Clough; Ichun Anderson Chen; Seong-Wook Park; Allison M Ahrens; Jeffrey N Stirman; Spencer L Smith; Jerry L Chen

doi:10.1038/s41467-021-26737-3

Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds

Nat Commun. 2021 Nov 17;12(1):6638. doi: 10.1038/s41467-021-26737-3.

Authors

Mitchell Clough¹, Ichun Anderson Chen^{1

2}, Seong-Wook Park^{1

2}, Allison M Ahrens³, Jeffrey N Stirman⁴, Spencer L Smith⁵, Jerry L Chen^{6

7

8}

Affiliations

¹ Department of Biomedical Engineering, Boston University, Boston, MA, 02215, USA.
² Center for Neurophotonics, Boston University, Boston, MA, 02215, USA.
³ Department of Biology, Boston University, Boston, MA, 02215, USA.
⁴ Neuroscience Center, University of North Carolina School of Medicine, Chapel Hill, NC, 27599, USA.
⁵ Department of Electrical and Computer Engineering, University of California Santa Barbara, Santa Barbara, CA, 93106, USA.
⁶ Department of Biomedical Engineering, Boston University, Boston, MA, 02215, USA. [email protected].
⁷ Center for Neurophotonics, Boston University, Boston, MA, 02215, USA. [email protected].
⁸ Department of Biology, Boston University, Boston, MA, 02215, USA. [email protected].

Abstract

Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Calcium / metabolism
Equipment Design
Mice
Microscopy, Fluorescence, Multiphoton / instrumentation*
Neurons / cytology
Neurons / physiology*
Optical Imaging / instrumentation*
Sensorimotor Cortex / cytology
Sensorimotor Cortex / physiology

Substances

Calcium

Abstract

Publication types

MeSH terms

Substances

Grants and funding