A protocol for the induction of experimental necrotizing enterocolitis in neonatal mice

STAR Protoc. 2021 Nov 11;2(4):100951. doi: 10.1016/j.xpro.2021.100951. eCollection 2021 Dec 17.

Abstract

Recapitulating human NEC using animal models has been insightful in dissecting the signaling pathways, immune-mediated mechanisms, genetic signatures, and the intestinal architecture of NEC. This protocol describes an in vivo murine NEC model, using hypoxia and formula containing lipopolysaccharide and enteric bacteria derived from an infant with NEC. With this mouse model, we aim to further dissect NEC pathogenesis and develop new therapeutic strategies. For complete details on the use and execution of this protocol, please refer to Mihi et al. (2021).

Keywords: Developmental biology; Health Sciences; Microbiology; Model Organisms; Molecular Biology.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Disease Models, Animal*
  • Enterocolitis, Necrotizing*
  • Feces / chemistry
  • Hypoxia / physiopathology
  • Ileum / pathology
  • Ileum / physiopathology
  • Lipopolysaccharides / adverse effects
  • Mice

Substances

  • Lipopolysaccharides