Use of a Ferret Model to Test Efficacy and Immunogenicity of Live Attenuated Mycobacterium avium Subspecies paratuberculosis Vaccines

John P Bannantine; Tuhina Gupta; Denise K Zinniel; Ahmed Hikal; Frederick D Quinn; Raul G Barletta

doi:10.1007/978-1-0716-1888-2_5

Use of a Ferret Model to Test Efficacy and Immunogenicity of Live Attenuated Mycobacterium avium Subspecies paratuberculosis Vaccines

Methods Mol Biol. 2022:2411:95-104. doi: 10.1007/978-1-0716-1888-2_5.

Authors

John P Bannantine¹, Tuhina Gupta², Denise K Zinniel³, Ahmed Hikal², Frederick D Quinn², Raul G Barletta³

Affiliations

¹ National Animal Disease Center, USDA-ARS, Ames, IA, USA. [email protected].
² University of Georgia, College of Veterinary Medicine, Athens, GA, USA.
³ University of Nebraska-Lincoln, School of Veterinary Medicine and Biomedical Sciences, Lincoln, NE, USA.

PMID: 34816400
DOI: 10.1007/978-1-0716-1888-2_5

Abstract

Native hosts for the bacterial agent that causes Johne's disease are ruminants, which include cattle, sheep and goats among others. These large animals are often too costly to be used in testing experimental vaccines. In this chapter, we provide detailed methods to use an inexpensive and more manageable animal host, the ferret, to test efficacy and immunogenicity of live-attenuated Mycobacterium avium subspecies paratuberculosis (MAP) mutant strains prior to consideration as vaccine candidates.

Keywords: Animal model; Ferrets; Johne’s disease; Mycobacterium; Paratuberculosis.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Bacterial Vaccines
Cattle
Ferrets
Mycobacterium avium subsp. paratuberculosis* / genetics
Paratuberculosis* / prevention & control
Sheep
Vaccines, Attenuated

Substances

Bacterial Vaccines
Vaccines, Attenuated