Biotechnological advancements have paved newer avenues for developing and designing novel and effective vaccines for rendering protection from various types of infectious diseases. Use of immunogenic genes via plasmid DNA constitutes an important next-generation biotechnological approach to fish immunization. In addition, the use of nanotechnology has significantly addressed the issue of mucosal mode of DNA vaccine delivery in aquaculture. Taking together both these advance technologies, this chapter entails a detailed protocol for the development of a nano-conjugated bicistronic DNA vaccine using chitosan nanoparticles as delivery vehicle, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene of Edwardsiella tarda as antigenic gene and interferon gamma (IFN-γ) gene of Labeo rohita as molecular adjuvant.
Keywords: Chitosan; DNA vaccine; Edwardsiella tarda; Nanoparticles.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.