CRISPR/Cas9 editing in conditionally immortalized HoxB8 cells for studying gene regulation in mouse dendritic cells

Huaming Xu; Thomas Look; Sujeethkumar Prithiviraj; Daniel Lennartz; Manuel Delgado Cáceres; Katrin Götz; Paul Wanek; Hans Häcker; Rafael Kramann; Kristin Seré; Martin Zenke

doi:10.1002/eji.202149482

CRISPR/Cas9 editing in conditionally immortalized HoxB8 cells for studying gene regulation in mouse dendritic cells

Eur J Immunol. 2022 Nov;52(11):1859-1862. doi: 10.1002/eji.202149482. Epub 2021 Dec 19.

Authors

Huaming Xu^{1

2}, Thomas Look^{1

2}, Sujeethkumar Prithiviraj^{1

2}, Daniel Lennartz^{1

2}, Manuel Delgado Cáceres^{1

2}, Katrin Götz^{1

2}, Paul Wanek^{1

2}, Hans Häcker³, Rafael Kramann⁴, Kristin Seré^{1

2}, Martin Zenke^{1

2}

Affiliations

¹ Department of Cell Biology, Institute of Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany.
² Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany.
³ Division of Microbiology and Immunology, Department of Pathology, University of Utah, Utah, USA.
⁴ Institute of Experimental Medicine and Systems Biology, RWTH Aachen University Medical School, Aachen, Germany.

PMID: 34826338
DOI: 10.1002/eji.202149482

Abstract

HoxB8 multipotent progenitors (MPP) are obtained by expression of the estrogen receptor hormone binding domain (ERHBD) HoxB8 fusion gene in mouse BM cells. HoxB8 MPP generate (i) the full complement of DC subsets (cDC1, cDC2, and pDC) in vitro and in vivo and (ii) allow CRISPR/Cas9-mediated gene editing, for example, generating homozygous deletions in cis-acting DNA elements at high precision, and (iii) efficient gene repression by dCas9-KRAB for studying gene regulation in DC differentiation.

Keywords: CRISPR/Cas; Dendritic cells; HoxB8; dCas9; gene regulation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CRISPR-Cas Systems*
Cell Line
Dendritic Cells
Gene Editing*
Gene Expression Regulation
Homeodomain Proteins / genetics
Mice

Substances

Hoxb8 protein, mouse
Homeodomain Proteins