We describe a protocol for the assembly and application of infrared (IR-B) laser-based set-ups to be used for localized heating of solid-supported planar and vesicular lipid membrane assemblies.
Keywords: Biomembranes; Giant unilamellar vesicle; IR-B laser; Laser-heating; Microthermocouple; Solid-supported lipid bilayers; Thermal gradient.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.