Biophoton imaging identification of delayed functional neural circuit injury after cerebral ischemia-reperfusion

Background: The evaluation of structural changes after stroke has made great progress; however, it remains difficult to evaluate functional neural changes.

New method: Here, we report a novel imaging technique that could monitor delayed functional neural circuit injury in an animal model of cerebral ischemia-reperfusion. The changes in 50 mM glutamate-induced biophotonic activities in functional neural circuits in rat brain slices after middle cerebral artery occlusion were investigated with an ultraweak biophoton imaging system.

Results: Six hours after ischemia-reperfusion, the rats presented a significant decrease in motion ability together with a large part of the unstained 2,3,5-Triphenyltetrazolium chloride (TTC) area in the ischemia-reperfusion side, whereas the intensity of the biophoton emissions was consistent on both the ischemia-reperfusion and non-ischemic sides of brain slices. Twenty-four hours after reperfusion, the behavior evaluation and TTC staining recovered slightly, and the intensity of the biophoton emissions was weaker on the ischemia-reperfusion side than on the contralateral side. One week after reperfusion, the behavioral test and TTC staining recovered to normal levels; however, the intensity of the biophoton emissions was decreased significantly on both the ischemia-reperfusion and contralateral sides, and such changes were even distinguished in different brain areas, such as the sensory and motor coteries and striatum.

Conclusion: These findings suggest that delayed functional neural circuit injury induced by cerebral ischemia-reperfusion could be identified with biophoton imaging techniques, providing a novel functional evaluation method for animal models of cerebral ischemia-reperfusion.