Abstract
The effect of recombinant human granulocyte colony-stimulating factor (G-CSF) on induction of differentiation of mouse myeloid leukemic M1 cells was examined. Purified G-CSF caused dose-dependent induction of phagocytic activity and lysozyme activity in M1 cells. Its half-maximally effective concentration was 10 ng/ml. On treatment of M1 cells with G-CSF (100 ng/ml) for 4 days, 30-50% of the cells differentiated morphologically into macrophage cells; 30-40% of the cells were blast cells and 20-30% of the cells were forms intermediate between blastic cells and mature macrophages.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Cell Differentiation
-
Cell Line
-
Colony-Stimulating Factors / pharmacology*
-
Glycoproteins / pharmacology
-
Granulocytes*
-
Growth Inhibitors*
-
Interleukin-6*
-
Leukemia Inhibitory Factor
-
Leukemia, Myeloid / pathology*
-
Leukemia, Myeloid / physiopathology
-
Lymphokines*
-
Macrophages / pathology
-
Mice
-
Muramidase / metabolism
-
Phagocytosis
-
Recombinant Proteins / pharmacology
Substances
-
Colony-Stimulating Factors
-
Glycoproteins
-
Growth Inhibitors
-
Interleukin-6
-
LIF protein, human
-
Leukemia Inhibitory Factor
-
Lif protein, mouse
-
Lymphokines
-
Recombinant Proteins
-
Muramidase