Elucidation of Gene Expression Patterns in the Craniofacial Tissues of Mouse Embryos by Wholemount In Situ Hybridization

Joshua B Studdert; Heidi Bildsoe; V Pragathi Masamsetti; Patrick P L Tam

doi:10.1007/978-1-0716-1847-9_3

Elucidation of Gene Expression Patterns in the Craniofacial Tissues of Mouse Embryos by Wholemount In Situ Hybridization

Methods Mol Biol. 2022:2403:33-42. doi: 10.1007/978-1-0716-1847-9_3.

Authors

Joshua B Studdert¹, Heidi Bildsoe², V Pragathi Masamsetti³, Patrick P L Tam³

Affiliations

¹ Embryology Unit, Children's Medical Research Institute, Westmead, NSW, Australia. [email protected].
² Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, VIC, Australia.
³ Embryology Unit, Children's Medical Research Institute, Westmead, NSW, Australia.

PMID: 34913114
DOI: 10.1007/978-1-0716-1847-9_3

Abstract

Analysis of animal models allows a deeper understanding of craniofacial development in health and diseases of humans. Wholemount in situ hybridization (WISH) is an informative technique to visualize gene expression in tissues across the developmental stages of embryos. The principle of WISH is based on the complementary binding (hybridization) of the DNA/RNA probe to the target transcript. The bound probe can then be visualized by an enzymatic color reaction to delineate the expression pattern of transcripts within a tissue. Here we describe an optimized method to perform in situ hybridization in mouse embryos.

Keywords: Expression pattern; In situ hybridization; Probe; Wholemount.

MeSH terms

Animals
Gene Expression
Gene Expression Profiling*
Gene Expression Regulation, Developmental*
In Situ Hybridization
Mice
RNA Probes

Substances

RNA Probes