Spatially resolved sampling of the human oral cavity for metabolic profiling

Alessio Ciurli; Rico J E Derks; Maximilian Liebl; Christine Ammon; Jacques Neefjes; Martin Giera

doi:10.1016/j.xpro.2021.101002

Spatially resolved sampling of the human oral cavity for metabolic profiling

STAR Protoc. 2021 Dec 8;2(4):101002. doi: 10.1016/j.xpro.2021.101002. eCollection 2021 Dec 17.

Authors

Alessio Ciurli^{1

2}, Rico J E Derks², Maximilian Liebl², Christine Ammon², Jacques Neefjes¹, Martin Giera²

Affiliations

¹ Oncode Institute and Department of Cell and Chemical Biology, Leiden University Medical Center, 2333 Leiden, ZA, the Netherlands.
² Center for Proteomics and Metabolomics, Leiden University Medical Center, 2333 Leiden, ZA, the Netherlands.

Abstract

Here, we present a spatially resolved sampling protocol for the oral human cavity aimed at untargeted metabolomics. We describe the spatial collection of salivary biospecimens, their preparation, and subsequent mass-spectrometry-based untargeted metabolomics analysis. Our protocol avoids complex procedures generally required for gland-specific saliva collection. For the human oral cavity, we provide an easy, flexible, and reproducible solution to comprehensively map the highly heterogeneous environment and elucidate the functionality of salivary components. For complete details on the use and execution of this protocol, please refer to Ciurli et al. (2021).

Keywords: Clinical Protocol; Health Sciences; Mass Spectrometry; Metabolism; Metabolomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Liquid
Humans
Mass Spectrometry / methods
Metabolome / physiology
Metabolomics / methods*
Mouth / chemistry*
Mouth / metabolism
Saliva / chemistry
Saliva / metabolism