Regulation of bcl-2 proto-oncogene expression during normal human lymphocyte proliferation

Science. 1987 Jun 5;236(4806):1295-9. doi: 10.1126/science.3495884.

Abstract

The bcl-2 and c-myc proto-oncogenes are brought into juxtaposition with the immunoglobulin heavy chain locus in particular B-cell lymphomas, resulting in high levels of constitutive accumulation of their messenger RNAs. Precisely how the products of the bcl-2 and c-myc genes contribute to tumorigenesis is unknown, but observations that c-myc expression is rapidly induced in nonneoplastic lymphocytes upon stimulation of proliferation raise the possibility that this proto-oncogene is involved in the control of normal cellular growth. In addition to c-myc, the bcl-2 proto-oncogene also was expressed in normal human B and T lymphocytes after stimulation with appropriate mitogens. Comparison of the regulation of the expression of these proto-oncogenes demonstrated marked differences and provided evidence that, in contrast to c-myc, levels of bcl-2 messenger RNA are regulated primarily through transcriptional mechanisms.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blood Proteins / biosynthesis
  • Blood Proteins / drug effects
  • Cycloheximide / pharmacology
  • Gene Expression Regulation / drug effects*
  • Humans
  • Interleukin-2 / pharmacology
  • Kinetics
  • Lymphocyte Activation / drug effects*
  • Phytohemagglutinins / pharmacology
  • Proto-Oncogene Mas
  • Proto-Oncogenes / drug effects*
  • RNA, Messenger / blood
  • RNA, Messenger / drug effects
  • Transcription, Genetic / drug effects

Substances

  • Blood Proteins
  • Interleukin-2
  • MAS1 protein, human
  • Phytohemagglutinins
  • Proto-Oncogene Mas
  • RNA, Messenger
  • Cycloheximide