Abstract
Conditioned medium from a T-lymphoblastic cell line (Mo) contains a number of well-characterized hemopoietins. In this paper we demonstrate that Mo cells also release a factor(s) able to stimulate the growth and the differentiation of megakaryocytic progenitors into large-size pure megakaryocytic colonies in plasma clot cultures. Comparison with other sources of human-active hemopoietins shows that Mo-conditioned medium performs better than others, especially for the megakaryocytic lineage. The factor(s) shows strong similarities with human Meg-CSF obtained from a thrombocytopenic patient's plasma, and is distinguishable from the other hemopoietins present in the medium.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bone Marrow Cells
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Cell Line
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Colony-Forming Units Assay
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Dose-Response Relationship, Drug
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GPI-Linked Proteins
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Humans
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Interleukin-3 / physiology
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Leukemia, Hairy Cell / metabolism
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Lymphocyte Activation / drug effects
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Lymphocyte Cooperation
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Megakaryocytes / cytology
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Megakaryocytes / drug effects*
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Membrane Glycoproteins
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Mesothelin
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Molecular Weight
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Phytohemagglutinins / pharmacology
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Protein Biosynthesis*
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Proteins*
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Purpura, Thrombocytopenic / pathology
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T-Lymphocytes / classification
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T-Lymphocytes / metabolism*
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Thrombocythemia, Essential / pathology
Substances
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GPI-Linked Proteins
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Interleukin-3
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Membrane Glycoproteins
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Phytohemagglutinins
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Proteins
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Mesothelin