Objective: To analyze the distribution and genetic characteristics of sporadic adult diarrhea virus in Chaoyang District, Beijing. Methods: Fecal samples from 177 adult patients with sporadic diarrhea were collected from 4 enteric outpatient clinics in Chaoyang District, Beijing from May to December 2019. Nucleic acid detection of Norovirus, Sappovirus, Rotavirus, Enteric Adenovirus and Astrovirus in the samples was performed by real-time quantitative PCR. The positive samples were amplified by RT-PCR/PCR and sequenced. The phylogenetic analysis was performed by neighbor-Joining (NJ) methods of Mega 6.0 software. Results: There were 60 of 177 (33.90%) adult sporadic diarrhea samples positive for enteric viral pathogens. Among them, 47 cases were infected with single virus, including 29 cases of Norovirus, 9 cases of Sappovirus, 8 cases of Astrovirus and 1 case of Enteric Adenovirus, in addition with 13 cases of multiple infections. None of rotavirus was detected. Partial sequences were successfully obtained for analysis, including 16 cases of GI Norovirus (7 subtypes and GI.3[P13] predominant), 10 cases of GII Norovirus (5 subtypes and GII.6[P7] predominant), 12 cases of Sappovirus (4 subtypes and GI.2 predominant), and 7 cases of Astrovirus (2 subtypes and AST-1 predominant). Conclusion: Norovirus, Astrovirus and Sappovirus are main pathogens among sporadic adult diarrhea in Beijing in 2019, and and different pathogenic gene subtypes show diverse characteristics.
目的： 分析2019年北京市成人散发性腹泻病毒病原的检出情况及其基因特征。 方法： 收集2019年5—12月北京市朝阳区4家肠道门诊送检的177例成人散发性腹泻粪便样本177份，应用实时荧光定量PCR技术对样本中的诺如病毒、札如病毒、轮状病毒、肠道腺病毒和星状病毒进行核酸检测，对阳性样本利用普通PCR技术进行病毒核酸扩增并测序，采用Mega 6.0软件的Neighbor-Joining法进行系统进化分析。 结果： 177份成人散发性腹泻样本中检测出60份病毒病原阳性（33.90%），其中47份检出单一病原，分别为诺如病毒29例、札如病毒9例、星状病毒8例和肠道腺病毒1例；13例患者样本检出两种病原；未检出轮状病毒。测序结果显示，诺如病毒GⅠ型测序成功16例，含7种基因亚型，主要为GⅠ.3［P13］亚型；诺如病毒GⅡ型测序成功10例，含5种基因亚型，以GⅡ.6［P7］亚型为主；札如病毒测序成功12例，含4种基因亚型，以GⅠ.2亚型为主；星状病毒测序成功7例，含2种基因亚型，以Ast-1亚型为主。 结论： 2019年北京市成人散发性腹泻病毒病原以诺如病毒、星状病毒和札如病毒为主，不同病原基因亚型呈现出多样性特征。.