Analysis of Micro-RNA-144 Expression Profile in Patients with Multiple Sclerosis in Comparison with Healthy Individuals

Fatemeh Roshani; Hossien Delavar Kasmaee; Kowsar Falahati; Ghazaleh Arabzade; Atefeh Sohan Forooshan Moghadam; Mohammad Hossein Sanati

doi:10.52547/rbmb.10.3.396

Analysis of Micro-RNA-144 Expression Profile in Patients with Multiple Sclerosis in Comparison with Healthy Individuals

Rep Biochem Mol Biol. 2021 Oct;10(3):396-401. doi: 10.52547/rbmb.10.3.396.

Authors

Fatemeh Roshani¹, Hossien Delavar Kasmaee², Kowsar Falahati³, Ghazaleh Arabzade¹, Atefeh Sohan Forooshan Moghadam⁴, Mohammad Hossein Sanati³

Affiliations

¹ Department of Genetics, Nourdanesh Institute of higher Education, Myme, Esfahan, Iran.
² medical university of Shahid Beheshti, Tehran.
³ Medical Genetic Department, National Institute of Genetics Engineering and Biothechnology, Tehran, Iran.
⁴ Department of Genetics, Nourdanesh Institute of higher Education, Myme, Esfahan,Iran.

Abstract

Background: Etiology of multiple sclerosis is non-clarified. It seems that environmental factors impact epigenetic in this disease. Micro-RNAs (MIR) as epigenetic factors are one of the most important factors in non-genetically neurodegenerative diseases. It has been found MIR-144 plays a main role in the regulation of many processes in the central nervous system. Here, we aimed to investigation of MIR-144 expression alteration in Multiple sclerosis (MS) patients.

Methods: In this study 32 healthy and 32 MS patient's blood sample were analyzed by quantitative Real-Time PCR method and obtained data analyzed by REST 2009 software.

Results: Analysis of Real-Time PCR data revealed that miR-144 Increase significantly in MS patients compared to healthy controls.

Conclusion: The increase of MIR-144 expression in MS patients is obvious. MIR-144 can be used as a biomarker of MS and help to early diagnosis and treatment of this disease.

Keywords: MiRNA-144; MicroRNA (miRNA); Multiple Sclerosis (MS).